Takemoto, N, Suehara, T, Frisco, HL, Sato, S-I, Sezaki, T, Kusamori, K, Kawazoe, Y, Park, SM, Yamazoe, S, Mizuhata, Y, Inoue, R, Miller, GJ ORCID: https://orcid.org/0000-0001-6533-3306, Hansen, SU, Jayson, GC, Gardiner, JM, Kanaya, T, Tokitoh, N, Ueda, K, Takakura, Y, Kioka, N, Nishikawa, M and Uesugi, M (2013) Small-Molecule-Induced Clustering of Heparan Sulfate Promotes Cell Adhesion. Journal Of The American Chemical Society, 135 (30). 11032 -11039.

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Abstract

Adhesamine is an organic small molecule that promotes adhesion and growth of cultured human cells by binding selectively to heparan sulfate on the cell surface. The present study combined chemical, physicochemical, and cell biological experiments, using adhesamine and its analogues, to examine the mechanism by which this dumbbell-shaped, non-peptidic molecule induces physiologically relevant cell adhesion. The results suggest that multiple adhesamine molecules cooperatively bind to heparan sulfate and induce its assembly, promoting clustering of heparan sulfate-bound syndecan-4 on the cell surface. A pilot study showed that adhesamine improved the viability and attachment of transplanted cells in mice. Further studies of adhesamine and other small molecules could lead to the design of assembly-inducing molecules for use in cell biology and cell therapy.

Item Type: Article
Subjects: Q Science > QD Chemistry
R Medicine > R Medicine (General)
Divisions: Faculty of Natural Sciences > School of Physical and Geographical Sciences
Related URLs:
Depositing User: Symplectic
Date Deposited: 26 Nov 2015 09:56
Last Modified: 24 May 2019 14:24
URI: http://eprints.keele.ac.uk/id/eprint/1225

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