MacLean, L and Myburgh, E and Rodgers, J and Price, HP (2013) Imaging African trypanosomes. Parasite Immunology, 35 (9/10). 283 -294. ISSN 1365-3024
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Trypanosoma brucei are extracellular kinetoplastid parasites transmitted by the blood-sucking tsetse fly. They are responsible for the fatal disease human African trypanosomiasis (HAT), also known as sleeping sickness. In late-stage infection, trypanosomes cross the blood-brain barrier (BBB) and invade the central nervous system (CNS) invariably leading to coma and death if untreated. There is no available vaccine and current late-stage HAT chemotherapy consists of either melarsoprol, which is highly toxic causing up to 8% of deaths, or nifurtimox-eflornithine combination therapy (NECT), which is costly and difficult to administer. There is therefore an urgent need to identify new late-stage HAT drug candidates. Here, we review how current imaging tools, ranging from fluorescent confocal microscopy of live immobilized cells in culture to whole-animal imaging, are providing insight into T. brucei biology, parasite-host interplay, trypanosome CNS invasion and disease progression. We also consider how imaging tools can be used for candidate drug screening purposes that could lead to new chemotherapies.
|Uncontrolled Keywords:||animal model, electron microscopy, in vivo imaging, RNA interference, tools and techniques, Trypanosoma spp|
|Subjects:||Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
|Divisions:||Faculty of Natural Sciences > School of Life Sciences|
|Date Deposited:||27 Nov 2015 15:03|
|Last Modified:||27 Nov 2015 15:03|
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