Kay, AG and Dale, TP and Akram, KM and Mohan, P and Hampson, K and Maffulli, N and Spiteri, MA and El Haj, AJ and Forsyth, NR (2015) BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation. Regen Med, 10 (2). 109 - 125. ISSN 1746-0751
N Forsyth - BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation.doc - Accepted Version
Available under License Creative Commons Attribution Non-commercial.
AIM: Human mesenchymal stem cells (hMSC) are multipotent progenitor cells. We propose the optimization of hMSC isolation and recovery using the application of a controlled hypoxic environment. MATERIALS & METHODS: We evaluated oxygen, glucose and serum in the recovery of hMSC from bone marrow (BMhMSC). Colony forming units-fibroblastic, cell numbers, tri-lineage differentiation, immunofluorescence and microarray were used to confirm and characterize BMhMSC. RESULTS: In an optimized (2% O(2), 4.5 g/l glucose and 5% serum) environment both colony forming units-fibroblastic (p = 0.01) and cell numbers (p = 0.0001) were enhanced over standard conditions. Transcriptional analysis identified differential expression of bone morphogenetic protein 2 (BMP2) and, putatively, chemokine (C-X-C motif) receptor 2 (CXCR2) signaling pathways. CONCLUSION: We have detailed a potential milestone in the process of refinement of the BMhMSC isolation process.
|Uncontrolled Keywords:||Mesenchymal stem cells, physiological normoxia, hypoxia, BMP2, transcriptome, CFU-F|
|Subjects:||Q Science > Q Science (General)
R Medicine > R Medicine (General)
|Divisions:||Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine|
|Date Deposited:||07 Jan 2016 09:02|
|Last Modified:||31 May 2016 11:03|
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