Wu, P and Farrell, W and Haworth, K and Emes, R and Kitchen, M and Glossop, J and Hanna, F and Fryer, A (2018) Maternal genome-wide DNA methylation profiling in gestational diabetes shows distinctive disease-associated changes relative to matched healthy pregnancies. Epigenetics, 13 (2). pp. 122-128. ISSN 1559-2308

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Abstract

Several recent reports have described associations between gestational diabetes (GDM) and changes to the epigenomic landscape where the DNA samples were derived from either cord or placental sources. We employed genome-wide 450K array analysis to determine changes to the epigenome in a unique cohort of maternal blood DNA from 11 pregnant women prior to GDM development relative to matched controls. Hierarchical clustering segregated the samples into 2 distinct clusters comprising GDM and healthy pregnancies. Screening identified 100 CpGs with a mean β-value difference of ≥0.2 between cases and controls. Using stringent criteria, 5 CpGs (within COPS8, PIK3R5, HAAO, CCDC124, and C5orf34 genes) demonstrated potentials to be clinical biomarkers as revealed by differential methylation in 8 of 11 women who developed GDM relative to matched controls. We identified, for the first time, maternal methylation changes prior to the onset of GDM that may prove useful as biomarkers for early therapeutic intervention.

Item Type: Article
Uncontrolled Keywords: gestational diabetes, epigenetics, fetal programming, biomarker, 450k array
Subjects: R Medicine > RC Internal medicine > RC648 Diseases of the endocrine glands. Clinical endocrinology.
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Depositing User: Symplectic
Date Deposited: 17 Mar 2016 14:24
Last Modified: 12 Apr 2018 15:52
URI: http://eprints.keele.ac.uk/id/eprint/1578

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