Foglia, F and Hazael, R and Simeoni, GG and Appavou, MS and Moulin, M and Haertlein, M and Trevor Forsyth, V and Seydel, T and Daniel, I and Meersman, F and McMillan, PF (2016) Water Dynamics in Shewanella oneidensis at Ambient and High Pressure using Quasi-Elastic Neutron Scattering. Scientific Reports, 6. 18862 -?. ISSN 2045-2322

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Abstract

Quasielastic neutron scattering (QENS) is an ideal technique for studying water transport and relaxation dynamics at pico- to nanosecond timescales and at length scales relevant to cellular dimensions. Studies of high pressure dynamic effects in live organisms are needed to understand Earth's deep biosphere and biotechnology applications. Here we applied QENS to study water transport in Shewanella oneidensis at ambient (0.1 MPa) and high (200 MPa) pressure using H/D isotopic contrast experiments for normal and perdeuterated bacteria and buffer solutions to distinguish intracellular and transmembrane processes. The results indicate that intracellular water dynamics are comparable with bulk diffusion rates in aqueous fluids at ambient conditions but a significant reduction occurs in high pressure mobility. We interpret this as due to enhanced interactions with macromolecules in the nanoconfined environment. Overall diffusion rates across the cell envelope also occur at similar rates but unexpected narrowing of the QENS signal appears between momentum transfer values Q = 0.7-1.1 Å(-1) corresponding to real space dimensions of 6-9 Å. The relaxation time increase can be explained by correlated dynamics of molecules passing through Aquaporin water transport complexes located within the inner or outer membrane structures.

Item Type: Article
Uncontrolled Keywords: Biological physics, Membrane biophysics
Subjects: Q Science > QC Physics
Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Natural Sciences > School of Physical and Geographical Sciences
Related URLs:
Depositing User: Symplectic
Date Deposited: 17 May 2016 15:37
Last Modified: 01 Jun 2016 15:25
URI: http://eprints.keele.ac.uk/id/eprint/1736

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