Davies, D, Heylings, JR, Gayes, H, McCarthy, T and Mack, MC (2016) Further development of an in vitro model for studying the penetration of chemicals through compromised skin. Toxicology in Vitro, 38. pp. 101-107. ISSN 1879-3177

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Abstract

A new in vitro model based on the electrical resistance properties of the skin barrier has been established in this laboratory. The model utilises a tape stripping procedure in dermatomed pig skin that removes a specific proportion of the stratum corneum, mimicking impaired barrier function observed in humans with damaged skin. The skin penetration and distribution of chemicals with differing physicochemical properties, namely; Benzoic acid, 3-Aminophenol, Caffeine and Sucrose has been assessed in this model. Although, skin penetration over 24 h differed for each chemical, compromising the skin did not alter the shape of the time course profile, although absorption into receptor fluid was higher for each chemical. Systemic exposure (receptor fluid, epidermis and dermis), was marginally higher in compromised skin following exposure to the fast penetrant, Benzoic acid, and the slow penetrant Sucrose. The systemically available dose of 3-Aminophenol increased to a greater extent and the absorption of Caffeine was more than double in compromised skin, suggesting that Molecular Weight and Log Pow, are not the only determinants for assessing systemic exposure under these conditions. Although further investigations are required, this in vitro model may be useful for prediction of dermal route exposure under conditions where skin barrier is impaired.

Item Type: Article
Additional Information: This is the accepted author manuscript (AAM). The final published version (version of record) is available online via Elsevier at https://doi.org/10.1016/j.tiv.2016.10.004 Please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: dermatomed pig skin; electrical resistance; barrier function; compromised skin; tape stripping; in vitro percutaneous absorption
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Medicine and Health Sciences > School of Pharmacy
Depositing User: Symplectic
Date Deposited: 17 Oct 2016 13:38
Last Modified: 18 Jun 2018 09:17
URI: http://eprints.keele.ac.uk/id/eprint/2281

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