Wiens, O and Xia, D and von Schubert, C and Wastling, JM and Dobbelaere, DA and Heussler, VT and Woods, KL (2014) Cell cycle-dependent phosphorylation of Theileria annulata schizont surface proteins. PLoS One, 9 (7). e103821 -?. ISSN 1932-6203

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Abstract

The invasion of Theileria sporozoites into bovine leukocytes is rapidly followed by the destruction of the surrounding host cell membrane, allowing the parasite to establish its niche within the host cell cytoplasm. Theileria infection induces host cell transformation, characterised by increased host cell proliferation and invasiveness, and the activation of anti-apoptotic genes. This process is strictly dependent on the presence of a viable parasite. Several host cell kinases, including PI3-K, JNK, CK2 and Src-family kinases, are constitutively activated in Theileria-infected cells and contribute to the transformed phenotype. Although a number of host cell molecules, including IkB kinase and polo-like kinase 1 (Plk1), are recruited to the schizont surface, very little is known about the schizont molecules involved in host-parasite interactions. In this study we used immunofluorescence to detect phosphorylated threonine (p-Thr), serine (p-Ser) and threonine-proline (p-Thr-Pro) epitopes on the schizont during host cell cycle progression, revealing extensive schizont phosphorylation during host cell interphase. Furthermore, we established a quick protocol to isolate schizonts from infected macrophages following synchronisation in S-phase or mitosis, and used mass spectrometry to detect phosphorylated schizont proteins. In total, 65 phosphorylated Theileria proteins were detected, 15 of which are potentially secreted or expressed on the surface of the schizont and thus may be targets for host cell kinases. In particular, we describe the cell cycle-dependent phosphorylation of two T. annulata surface proteins, TaSP and p104, both of which are highly phosphorylated during host cell S-phase. TaSP and p104 are involved in mediating interactions between the parasite and the host cell cytoskeleton, which is crucial for the persistence of the parasite within the dividing host cell and the maintenance of the transformed state.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via Public Library of Science at http://dx.doi.org/10.1371/journal.pone.0103821 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: Amino Acid Sequence, Animals, Antigens, Protozoan, Cattle, Cell Line, Cytokinesis, Host-Parasite Interactions, Interphase, Membrane Proteins, Phosphorylation, Protein Processing, Post-Translational, Protozoan Proteins, Schizonts, Theileria annulata
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Natural Sciences > School of Life Sciences
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Depositing User: Symplectic
Date Deposited: 19 Oct 2016 15:59
Last Modified: 12 Jul 2017 10:58
URI: http://eprints.keele.ac.uk/id/eprint/2287

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