Jenkins, SI and Roach, P and Chari, DM (2015) Development of a nanomaterial bio-screening platform for neurological applications. Nanomedicine: Nanotechnology, Biology and Medicine, 11 (1). 77 - 87. ISSN 1549-9634

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Abstract

Nanoparticle platforms are being intensively investigated for neurological applications. Current biological models used to identify clinically relevant materials have major limitations, e.g. technical/ethical issues with live animal experimentation, failure to replicate neural cell diversity, limited control over cellular stoichiometries and poor reproducibility. High-throughput neuro-mimetic screening systems are required to address these challenges. We describe an advanced multicellular neural model comprising the major non-neuronal/glial cells of the central nervous system (CNS), shown to account for ~99.5% of CNS nanoparticle uptake. This model offers critical advantages for neuro-nanomaterials testing while reducing animal use: one primary source and culture medium for all cell types, standardized biomolecular corona formation and defined/reproducible cellular stoichiometry. Using dynamic time-lapse imaging, we demonstrate in real-time that microglia (neural immune cells) dramatically limit particle uptake in other neural subtypes (paralleling post-mortem observations after nanoparticle injection in vivo), highlighting the utility of the system in predicting neural handling of biomaterials.

Item Type: Article
Uncontrolled Keywords: biomaterials screening, glia, multicellular models, neural cells, protein corona, animal use alternatives, animals, astrocytes, biocompatible materials, central nervous system, coculture techniques, culture media, croglia, microscopy, fluorescence, nanomedicine, nanoparticles, nanostructures, neuroglia, neurons, oligodendroglia, reproducibility of results, spectroscopy, fourier transform infrared
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 25 Oct 2016 08:37
Last Modified: 25 Oct 2016 08:37
URI: http://eprints.keele.ac.uk/id/eprint/2334

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