Jenkins, SI and Pickard, MR and Furness, DN and Yiu, HH and Chari, DM (2013) Differences in magnetic particle uptake by CNS neuroglial subclasses: implications for neural tissue engineering. Nanomedicine, 8 (6). 951 -968. ISSN 1748-6963

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Jenkins Pickard Furness Yiu Chari, 2013, Differences Sphero, NanomedLond.pdf - Accepted Version
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Abstract

AIM: To analyze magnetic particle uptake and intracellular processing by the four main non-neuronal subclasses of the CNS: oligodendrocyte precursor cells; oligodendrocytes; astrocytes; and microglia. MATERIALS & METHODS: Magnetic particle uptake and processing were studied in rat oligodendrocyte precursor cells and oligodendrocytes using fluorescence and transmission electron microscopy, and the results collated with previous data from rat microglia and astrocyte studies. All cells were derived from primary mixed glial cultures. RESULTS: Significant intercellular differences were observed between glial subtypes: microglia demonstrate the most rapid/extensive particle uptake, followed by astrocytes, with oligodendrocyte precursor cells and oligodendrocytes showing significantly lower uptake. Ultrastructural analyses suggest that magnetic particles are extensively degraded in microglia, but relatively stable in other cells. CONCLUSION: Intercellular differences in particle uptake and handling exist between the major neuroglial subtypes. This has important implications for the utility of the magnetic particle platform for neurobiological applications including genetic modification, transplant cell labeling and biomolecule delivery to mixed CNS cell populations.

Item Type: Article
Additional Information: © Future Medicine, this is the accepted manuscript of a paper published as Jenkins, S.I. et al., 2013. Differences in magnetic particle uptake by CNS neuroglial subclasses: implications for neural tissue engineering. Nanomedicine, 8(6), pp.951–968 and available at: http://dx.doi.org/10.2217/nnm.12.145
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
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Depositing User: Symplectic
Date Deposited: 25 Oct 2016 08:52
Last Modified: 25 Oct 2016 08:52
URI: http://eprints.keele.ac.uk/id/eprint/2336

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