Wright, KT and Seabright, R and Logan, A and Lilly, AJ and Khanim, F and Bunce, CM and Johnson, WEB (2010) Extracellular Nm23H1 stimulates neurite outgrowth from dorsal root ganglia neurons in vitro independently of nerve growth factor supplementation or its nucleoside diphosphate kinase activity. Biochemical and Biophysical Research Communications, 398 (1). 79 - 85. ISSN 1090-2104

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Abstract

The nucleoside diphosphate (NDP) kinase, Nm23H1, is a highly expressed during neuronal development, whilst induced over-expression in neuronal cells results in increased neurite outgrowth. Extracellular Nm23H1 affects the survival, proliferation and differentiation of non-neuronal cells. Therefore, this study has examined whether extracellular Nm23H1 regulates nerve growth. We have immobilised recombinant Nm23H1 proteins to defined locations of culture plates, which were then seeded with explants of embryonic chick dorsal root ganglia (DRG) or dissociated adult rat DRG neurons. The substratum-bound extracellular Nm23H1 was stimulatory for neurite outgrowth from chick DRG explants in a concentration-dependent manner. On high concentrations of Nm23H1, chick DRG neurite outgrowth was extensive and effectively limited to the location of the Nm23H1, i.e. neuronal growth cones turned away from adjacent collagen-coated substrata. Nm23H1-coated substrata also significantly enhanced rat DRG neuronal cell adhesion and neurite outgrowth in comparison to collagen-coated substrata. These effects were independent of NGF supplementation. Recombinant Nm23H1 (H118F), which does not possess NDP kinase activity, exhibited the same activity as the wild-type protein. Hence, a novel neuro-stimulatory activity for extracellular Nm23H1 has been identified in vitro, which may function in developing neuronal systems.

Item Type: Article
Uncontrolled Keywords: animals, cells, cultured, chick embryo, collagen type i, extracellular activity, ganglia, spinal, male, nerve growth, nerve growth factor, neurites, neurons, nm23 nucleoside diphosphate kinases, nm23h1, nucleoside diphosphate kinase, rats, rats, sprague-dawley, recombinant proteins
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 15 Nov 2016 15:02
Last Modified: 15 Nov 2016 15:02
URI: http://eprints.keele.ac.uk/id/eprint/2468

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