Wright, KT and Griffiths, GJ and Johnson, WEB (2010) A comparison of high-content screening versus manual analysis to assay the effects of mesenchymal stem cell-conditioned medium on neurite outgrowth in vitro. Journal of Biomolecular Screening, 15 (5). 576 - 582. ISSN 1552-454X

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Abstract

Bone marrow mesenchymal stem cells (MSCs) promote nerve growth and functional recovery in animal models of spinal cord injury (SCI) to varying levels. The authors have tested high-content screening to examine the effects of MSC-conditioned medium (MSC-CM) on neurite outgrowth from the human neuroblastoma cell line SH-SY5Y and from explants of chick dorsal root ganglia (DRG). These analyses were compared to previously published methods that involved hand-tracing individual neurites. Both methods demonstrated that MSC-CM promoted neurite outgrowth. Each showed the proportion of SH-SY5Y cells with neurites increased by ~200% in MSC-CM within 48 h, and the number of neurites/SH-SY5Y cells was significantly increased in MSC-CM compared with control medium. For high-content screening, the analysis was performed within minutes, testing multiple samples of MSC-CM and in each case measuring >15,000 SH-SY5Y cells. In contrast, the manual measurement of neurite outgrowth from >200 SH-SY5Y cells in a single sample of MSC-CM took at least 1 h. High-content analysis provided additional measures of increased neurite branching in MSC-CM compared with control medium. MSC-CM was also found to stimulate neurite outgrowth in DRG explants using either method. The application of the high-content analysis was less well optimized for measuring neurite outgrowth from DRG explants than from SH-SY5Y cells.

Item Type: Article
Additional Information: © The authors, Sage Publications The published version Wright, K.T., Griffiths, G.J. & Johnson, W.E.B., 2010. A Comparison of High-Content Screening versus Manual Analysis to Assay the Effects of Mesenchymal Stem Cell-Conditioned Medium on Neurite Outgrowth In Vitro. Journal of Biomolecular Screening, 15(5), pp.576–582, is available from Sage publishers http://dx.doi.org/10.1177/1087057110367959
Uncontrolled Keywords: algorithms, animals, cell line, tumor, chick embryo, culture media, conditioned, high-content screening, humans, manual image analysis, mesenchymal stem cell-conditioned medium, mesenchymal stromal cells, neurite outgrowth, neurites
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
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Depositing User: Symplectic
Date Deposited: 15 Nov 2016 15:07
Last Modified: 29 Jun 2017 15:15
URI: http://eprints.keele.ac.uk/id/eprint/2469

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