Whatmore, A, Koylass, M, Muchowski, J, Edwards-Smallbone, J and Gopaul, K (2016) Extended Multilocus Sequence Analysis to Describe the Global Population Structure of the Genus Brucella: Phylogeography and Relationship to Biovars. Frontiers in Microbiology, 7. ISSN 1664-302X

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Abstract

An extended multilocus sequence analysis (MLSA) scheme applicable to the Brucella, an expanding genus that includes zoonotic pathogens that severely impact animal and human health across large parts of the globe, was developed. The scheme, which extends a previously described nine locus scheme by examining sequences at 21 independent genetic loci in order to increase discriminatory power, was applied to a globally and temporally diverse collection of over 500 isolates representing all 12 known Brucella species providing an expanded and detailed understanding of the population genetic structure of the group. Over 100 sequence types (STs) were identified and analysis of data provided insights into both the global evolutionary history of the genus, suggesting that early emerging Brucella abortus lineages might be confined to Africa while some later lineages have spread worldwide, and further evidence of the existence of lineages with restricted host or geographical ranges. The relationship between biovar, long used as a crude epidemiological marker, and genotype was also examined and showed decreasing congruence in the order Brucella suis > B. abortus > Brucella melitensis

Item Type: Article
Additional Information: This is the accepted author manuscript (AAM). The final published version (version of record) is available online via Frontiers Media at https://doi.org/10.3389/fmicb.2016.02049 Please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: Brucella, brucellosis; multilocus sequence; molecular typing; zoonosis
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Natural Sciences > School of Life Sciences
Depositing User: Symplectic
Date Deposited: 04 Jan 2017 11:38
Last Modified: 15 Jun 2018 10:56
URI: http://eprints.keele.ac.uk/id/eprint/2716

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