Morganstein, DL and Wu, P and Mane, MR and Fisk, NM and White, R and Parker, MG (2010) Human fetal mesenchymal stem cells differentiate into brown and white adipocytes: a role for ERRalpha in human UCP1 expression. Cell Research, 20 (4). 434 -444. ISSN 1748-7838

[img]
Preview
Text
Human fetal mesenchymal stem cells differentiate into brown and white adipocytes: a role for ERRalpha in human UCP1 expression.pdf - Accepted Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview

Abstract

We investigated the ability of fetal mesenchymal stem cells (fMSCs) to differentiate into brown and white adipocytes and compared the expression of a number of marker genes and key regulatory factors. We showed that the expression of key adipocyte regulators and markers during differentiation is similar to that in other human and murine adipocyte models, including induction of PPARgamma2 and FABP4. Notably, we found that the preadipocyte marker, Pref-1, is induced early in differentiation and then declines markedly as the process continues, suggesting that fMSCs first acquire preadipocyte characteristics as they commit to the adipogenic lineage, prior to their differentiation into mature adipocytes. After adipogenic induction, some stem cell isolates differentiated into cells resembling brown adipocytes and others into white adipocytes. Detailed investigation of one isolate showed that the novel brown fat-determining factor PRDM16 is expressed both before and after differentiation. Importantly, these cells exhibited elevated basal UCP-1 expression, which was dependent on the activity of the orphan nuclear receptor ERRalpha, highlighting a novel role for ERRalpha in human brown fat. Thus fMSCs represent a useful in vitro model for human adipogenesis, and provide opportunities to study the stages prior to commitment to the adipocyte lineage. They also offer invaluable insights into the characteristics of human brown fat.

Item Type: Article
Uncontrolled Keywords: Adipocytes, Brown, White, Animal, Cell Differentiation, DNA-Binding Proteins, Fatty Acid-Binding Proteins, Fetus, Humans, Intercellular Signaling Peptides and Proteins, Ion Channels, Membrane Proteins, Mesenchymal Stromal Cells, Mice, Mitochondrial Proteins, PPAR gamma, Receptors, Estrogen, Transcription Factors, Uncoupling Protein 1
Subjects: Q Science > QD Chemistry > QD415 Biochemistry
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 19 Jan 2017 09:19
Last Modified: 19 Jan 2017 09:19
URI: http://eprints.keele.ac.uk/id/eprint/2786

Actions (login required)

View Item View Item