Tickle, JA and Jenkins, SI and Polyak, B and Pickard, MR and Chari, DM (2016) Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance. Nanomedicine, 11 (4). 345 - 358. ISSN 1748-6963

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Abstract

AIM: To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles. MATERIALS & METHODS: MPs of varying magnetite content were applied to primary-derived rat cortical astrocytes ± static/oscillating magnetic fields to assess labeling efficiency and safety. RESULTS: Higher magnetite content particles display high but safe accumulation in astrocytes, with longer-term label retention versus lower/no magnetite content particles. Magnetic fields enhanced loading extent. Dynamic live cell imaging of dividing labeled astrocytes demonstrated that particle distribution into daughter cells is predominantly 'asymmetric'. CONCLUSION: These findings could inform protocols to achieve efficient MP loading into neural transplant cells, with significant implications for post-transplantation tracking/localization.

Item Type: Article
Additional Information: This work is made available online in accordance with publishers policy
Subjects: ?? Animals ??
?? Astrocytes ??
?? Cell Division ??
?? Cells, Cultured ??
?? Endocytosis ??
?? Magnetite Nanoparticles ??
?? Microscopy, Fluorescence ??
Q Science > Q Science (General)
R Medicine > R Medicine (General)
?? Rats ??
?? Rats, Sprague-Dawley ??
?? astrocytes ??
?? cell transplantation ??
?? label dilution ??
?? magnetite ??
?? magnetolabeling ??
?? polymeric particles ??
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 23 Jan 2017 11:16
Last Modified: 01 Feb 2017 01:30
URI: http://eprints.keele.ac.uk/id/eprint/2809

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