Rowe, M and Young, LS and Crocker, J and Stokes, H and Henderson, S and Rickinson, AB (1991) Epstein-Barr virus (EBV)-associated lymphoproliferative disease in the SCID mouse model: implications for the pathogenesis of EBV-positive lymphomas in man. Journal of Experimental Medicine, 173 (1). 147 - 158. ISSN 0022-1007

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Epstein-Barr virus (EBV)-associated lymphoproliferative disease in the SCID mouse model: implications for the pathogenesis of EBV-positive lymphomas in man.pdf - Published Version
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Abstract

When human peripheral blood lymphocytes (PBLs) from Epstein-Barr virus (EBV)-seropositive donors are injected intraperitoneally into SCID mice, EBV+ B cell tumors develop within weeks. A preliminary report (Mosier, D. E., R. J. Gulizia, S. M. Baird, D. D. Richman, D. B. Wilson, R. I. Fox, and T. J. Kipps, 1989. Blood. 74(Suppl. 1):52a) has suggested that such tumors resemble the EBV-positive malignancy, Burkitt's lymphoma. The present work shows that generally the human (hu) PBL-SCID tumors are distinct from Burkitt's lymphoma and instead resemble lymphoblastoid cell lines (LCLs) generated by EBV-infection of normal B cells in vitro in terms of: (a) their cell surface phenotype, with expression of B cell activation antigens and adhesion molecules, (b) normal karyotype, and (c) viral phenotype, with expression of all the transformation-associated EBV latent proteins and, in a minority of cells, productive cycle antigens. Indeed, in vitro-transformed LCLs also grow when inoculated into SCID mice, the frequency of tumor outgrowth correlating with the in vitro growth phenotype of the LCL which is itself determined by the identity of the transforming virus (i.e., type 1 or type 2 EBV). Histologically the PBL-derived hu-SCID tumors resemble the EBV+ large cell lymphomas that develop in immuno-suppressed patients and, like the human tumors, often present at multiple sites as individual monoclonal or oligoclonal foci. The remarkable efficiency of tumor development in the hu-SCID model suggests that lymphomagenesis involves direct outgrowth of EBV-transformed B cells without requirement for secondary genetic changes, and that selection on the basis of cell growth rate alone is sufficient to explain the monoclonal/oligoclonal nature of tumor foci. EBV+ large cell lymphoma of the immunosuppressed may arise in a similar way.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via Rockefeller University Press at http://dx.doi.org/10.1084/jem.173.1.147 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: Animals, Antigens, Surface, Antigens, Viral, Cell Line, Chimera, Gene Expression, Herpesvirus 4, Humans, Immunologic Deficiency Syndromes, Lymphoma, Lymphoproliferative Disorders, Mice, Phenotype, Tumor Virus Infections,
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
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Depositing User: Symplectic
Date Deposited: 25 Jan 2017 09:28
Last Modified: 28 Jul 2017 13:33
URI: http://eprints.keele.ac.uk/id/eprint/2816

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