Jordan, A and Jacques, M and Merrick, C and Devos, J and Forsyth, VT and Porcar, L and Martel, A (2016) SEC-SANS: size exclusion chromatography combined in situ with small-angle neutron scattering. Journal of Applied Crystallography, 49 (Pt 6). 2015 - 2020. ISSN 1600-5767

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Abstract

The first implementation and use of an in situ size exclusion chromatography (SEC) system on a small-angle neutron scattering instrument (SANS) is described. The possibility of deploying such a system for biological solution scattering at the Institut Laue-Langevin (ILL) has arisen from the fact that current day SANS instruments at ILL now allow datasets to be acquired using small sample volumes with exposure times that are often shorter than a minute. This capability is of particular importance for the study of unstable biological macromolecules where aggregation or denaturation issues are a major problem. The first use of SEC-SANS on ILL's instrument D22 is described for a variety of proteins including one particularly aggregation-prone system.

Item Type: Article
Uncontrolled Keywords: SEC-SANS; size exclusion chromatography; small-angle neutron scattering; in situ; sample environment
Subjects: ?? SEC-SANS ??
?? in situ ??
?? sample environment ??
?? size exclusion chromatography ??
?? small-angle neutron scattering ??
Divisions: Faculty of Natural Sciences > School of Life Sciences
Related URLs:
Depositing User: Symplectic
Date Deposited: 20 Feb 2017 16:38
Last Modified: 01 May 2017 09:19
URI: http://eprints.keele.ac.uk/id/eprint/2928

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