Al-Dulaimi, Omar Ahmed Abass (2017) Phytochemical Composition and Antimicrobial Properties of Four Plants Indicated for Traditional Medicine Use. Doctoral thesis, Keele University.

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Abstract

The rapid development of seriously drug-resistant pathogen strains has created a dangerous problem to public health. The discovery of new effective antimicrobials remains an urgent task to control microbial resistance. Natural products can offer special stereochemistry and unlimited diversity of natural leads which are biologically active or ready for development and structure optimization strategies. Four medicinal plants Cylicodiscus gabunensis (CG), Pogostemon cablin (PC), Perilla frutescens (PF) and Magnolia biondii (MB) were selected for investigation of their phytochemical composition and antiplasmodial and/or antibacterial properties. A bioassay guided fractionation method has been followed to characterize the antiplasmodial and antibacterial constituents of CG. Flash column chromatography and preparative HPLC were used to obtain the bioactive compounds. GC-MS, LC-MS, and NMR have been used for chemical analysis purposes. For evaluation of the antibacterial activity, disk diffusion assay, alamar blue microplate assay, time kill kinetic and scanning electron microscopy (SEM) methods have been used. The work led to the isolation of the most potent antiplasmodial fraction of CG with IC50 of 4.7

Item Type: Thesis (Doctoral)
Subjects: R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Depositing User: Lisa Bailey
Date Deposited: 27 Mar 2017 11:30
Last Modified: 27 Mar 2017 11:30
URI: http://eprints.keele.ac.uk/id/eprint/3078

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