Ismail, Y, Wimpenny, I, Bretcanu, O, Dalgarno, K and El Haj, AJ (2017) Development of multi-substituted hydroxyapatite nanopowders as biomedical materials for bone tissue engineering applications. Journal of Biomedical Materials Research Part A, 105 (6). pp. 1775-1785. ISSN 1552-4965

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A El Haj - Development of multi-substituted hydroxyapatite nanopowers as biomedical materials for bone tissue engineering applications.pdf - Accepted Version

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Abstract

Ionic substitutions have been proposed as a tool to control the functional behavior of synthetic hydroxyapatite (HA), particularly for Bone Tissue Engineering applications. The effect of simultaneous substitution of different levels of carbonate (CO3) and silicon (Si) ions in the HA lattice was investigated. Furthermore, human bone marrow‐derived mesenchymal stem cells (hMSCs) were cultured on multi‐substituted HA (SiCHA) to determine if biomimetic chemical compositions were osteoconductive. Of the four different compositions investigates, SiCHA‐1 (0.58 wt % Si) and SiCHA‐2 (0.45 wt % Si) showed missing bands for CO3 and Si using FTIR analysis, indicating competition for occupation of the phosphate site in the HA lattice; 500°C was considered the most favorable calcination temperature as: (i) the powders produced possessed a similar amount of CO3 (2–8 wt %) and Si (<1.0 wt %) as present in native bone; and (ii) there was a minimal loss of CO3 and Si from the HA structure to the surroundings during calcination. Higher Si content in SiCHA‐1 led to lower cell viability and at most hindered proliferation, but no toxicity effect occurred. While, lower Si content in SiCHA‐2 showed the highest ALP/DNA ratio after 21 days culture with hMSCs, indicating that the powder may stimulate osteogenic behavior to a greater extent than other powders

Item Type: Article
Additional Information: This is the accepted author manuscript (AAM). The final published version (version of record) is available online via Wiley at https://doi.org/10.1002/jbm.a.36038 Please refer to any applicable terms of use of the publisher.
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Depositing User: Symplectic
Date Deposited: 27 Mar 2017 14:18
Last Modified: 12 Jun 2018 10:13
URI: http://eprints.keele.ac.uk/id/eprint/3088

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