Hu, B and El Haj, AJ and Dobson, J (2013) Receptor-targeted, magneto-mechanical stimulation of osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. International Journal of Molecular Sciences, 14 (9). 19276 - 19293. ISSN 1422-0067

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Abstract

Mechanical cues are employed to promote stem cell differentiation and functional tissue formation in tissue engineering and regenerative medicine. We have developed a Magnetic Force Bioreactor (MFB) that delivers highly targeted local forces to cells at a pico-newton level, utilizing magnetic micro- and nano-particles to target cell surface receptors. In this study, we investigated the effects of magnetically targeting and actuating specific two mechanical-sensitive cell membrane receptors-platelet-derived growth factor receptor α (PDGFRα) and integrin ανβ3. It was found that a higher mineral-to-matrix ratio was obtained after three weeks of magneto-mechanical stimulation coupled with osteogenic medium culture by initially targeting PDGFRα compared with targeting integrin ανβ3 and non-treated controls. Moreover, different initiation sites caused a differentiated response profile when using a 2-day-lagged magneto-mechanical stimulation over culture periods of 7 and 12 days). However, both resulted in statistically higher osteogenic marker genes expression compared with immediate magneto-mechanical stimulation. These results provide insights into important parameters for designing appropriate protocols for ex vivo induced bone formation via magneto-mechanical actuation.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via MDPI at http://dx.doi.org/10.3390/ijms140919276 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: bone marrow cells, cell differentiation, cell membrane, cells, cultured, humans, integrin alphavbeta3, magnetic fields, magnetite nanoparticles, mechanotransduction, cellular, mesenchymal stromal cells, oligopeptides, osteogenesis, receptor, platelet-derived growth factor alpha, spectroscopy, fourier transform infrared
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 12 Apr 2017 10:00
Last Modified: 12 Apr 2017 10:01
URI: http://eprints.keele.ac.uk/id/eprint/3226

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