Roach, P and McGarvey, DJ and Lees, MR and Hoskins, C (2013) Remotely triggered scaffolds for controlled release of pharmaceuticals. International Journal of Molecular Sciences, 14 (4). 8585 -8602. ISSN 1422-0067

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Abstract

Fe3O4-Au hybrid nanoparticles (HNPs) have shown increasing potential for biomedical applications such as image guided stimuli responsive drug delivery. Incorporation of the unique properties of HNPs into thermally responsive scaffolds holds great potential for future biomedical applications. Here we successfully fabricated smart scaffolds based on thermo-responsive poly(N-isopropylacrylamide) (pNiPAM). Nanoparticles providing localized trigger of heating when irradiated with a short laser burst were found to give rise to remote control of bulk polymer shrinkage. Gold-coated iron oxide nanoparticles were synthesized using wet chemical precipitation methods followed by electrochemical coating. After subsequent functionalization of particles with allyl methyl sulfide, mercaptodecane, cysteamine and poly(ethylene glycol) thiol to enhance stability, detailed biological safety was determined using live/dead staining and cell membrane integrity studies through lactate dehydrogenase (LDH) quantification. The PEG coated HNPs did not show significant cytotoxic effect or adverse cellular response on exposure to 7F2 cells (p < 0.05) and were carried forward for scaffold incorporation. The pNiPAM-HNP composite scaffolds were investigated for their potential as thermally triggered systems using a Q-switched Nd:YAG laser. These studies show that incorporation of HNPs resulted in scaffold deformation after very short irradiation times (seconds) due to internal structural heating. Our data highlights the potential of these hybrid-scaffold constructs for exploitation in drug delivery, using methylene blue as a model drug being released during remote structural change of the scaffold.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via MDPI at http://dx.doi.org/10.3390/ijms14048585 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: scaffold, smart material, thermo-responsive, hybrid nanoparticle, surface plasmon
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 12 Apr 2017 11:28
Last Modified: 12 Apr 2017 11:29
URI: http://eprints.keele.ac.uk/id/eprint/3236

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