Lim, J and Clements, MA and Dobson, J (2012) Delivery of short interfering ribonucleic acid-complexed magnetic nanoparticles in an oscillating field occurs via caveolae-mediated endocytosis. PLoS One, 7 (12). e51350 -?. ISSN 1932-6203

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Abstract

Gene delivery technologies to introduce foreign genes into highly differentiated mammalian cells have improved significantly over the last few decades. Relatively new techniques such as magnetic nanoparticle-based gene transfection technology are showing great promise in terms of its high transfection efficiency and wide-ranging research applications. We have developed a novel gene delivery technique, which uses magnetic nanoparticles moving under the influence of an oscillating magnetic array. Herein we successfully introduced short interfering RNA (siRNA) against green fluorescent protein (GFP) or actin into stably-transfected GFP-HeLa cells or wild-type HeLa and rat aortic smooth muscle cells, respectively. This gene silencing technique occurred in a dose- and cell density- dependent manner, as reflected using fluorescence intensity and adhesion assays. Furthermore, using endocytosis inhibitors, we established that these magnetic nanoparticle-nucleic acid complexes, moving across the cell surface under the influence of an oscillating magnet array, enters into the cells via the caveolae-mediated endocytic pathway.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via Public Library of Science at http://dx.doi.org/10.1371/journal.pone.0051350 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: aged, aged, 80 and over, Alzheimer's Disease, cadaver, female, hippocampus, human, humans, iron, magnetic resonance imaging, male, microscopy, MRI, reproducibility of results, sensitivity and specificity, statistics as topic, tissue distribution, variance
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 12 Apr 2017 11:38
Last Modified: 12 Apr 2017 11:43
URI: http://eprints.keele.ac.uk/id/eprint/3239

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