Hasenkamp, S and Wong, EH and Horrocks, P (2012) An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum. Malaria Journal, 11. 42 -?. ISSN 1475-2875

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Abstract

This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.

Item Type: Article
Uncontrolled Keywords: luciferase, single-step lysis, stage-specific, promoter function, pcna
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
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Depositing User: Symplectic
Date Deposited: 09 Mar 2015 16:12
Last Modified: 06 Jul 2017 15:58
URI: http://eprints.keele.ac.uk/id/eprint/327

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