Hasenkamp, S and Russell, KT and Horrocks, P (2012) Comparison of the absolute and relative efficiencies of electroporation-based transfection protocols for Plasmodium falciparum. Malaria Journal, 11. 210 - ?. ISSN 1475-2875

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Abstract

BACKGROUND: Several electroporation protocols exist to transfect exogenous DNA into Plasmodium falciparum. To date, however, only a subjective analysis of their relative efficiencies has been reported. METHODS: A time-course of luciferase reporter expression is used to provide an objective quantitative analysis of the absolute efficiency of three electroporation techniques; direct electroporation of ring stage infected erythrocytes, preloading of erythrocytes and a novel "double-tap" protocol that combines both approaches. RESULTS: Preloading of erythrocytes shows a mean efficiency of 9.59 x 10(-6), some 5-180 fold more efficient than matched experiments utilizing the "double-tap" and direct electroporation of ring stage infected erythrocytes alone, respectively. CONCLUSION: Evidence presented here provides the first quantitative assessment of both the absolute and relative efficiencies of a key molecular tool used to study the biology and pathogenesis of this important human pathogen.

Item Type: Article
Uncontrolled Keywords: luciferase, electroporation, transfection efficiency, malaria
Subjects: R Medicine > R Medicine (General)
Related URLs:
Depositing User: Symplectic
Date Deposited: 09 Mar 2015 16:18
Last Modified: 13 Jul 2017 10:35
URI: http://eprints.keele.ac.uk/id/eprint/329

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