El-Faham, MH and Wheatcroft-Francklow, KJ and Price, HP and Sayers, JR and Doenhoff, MJ (2017) Schistosoma mansoni cercarial elastase (SmCE): differences in immunogenic properties of native and recombinant forms. Parasitology. ISSN 0031-1820

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Abstract

The Schistosoma mansoni cercarial elastase (SmCE) has previously been shown to be poorly immunogenic in mice. However, a minority of mice were able to produce antibodies against SmCE after multiple immunizations with crude preparations containing the enzyme. These mice were partially protected against challenge infections of S. mansoni. In the present study, we show that in contrast to the poor immunogenicity of the enzymatically active native form of SmCE derived from a crude preparation (cercarial transformation fluid), immunization of CBA/Ca mice with two enzymatically inactive forms, namely purified native SmCE or a recombinant SmCE fused to recombinant Schistosoma japonicum glutathione S-transferase (rSmCE-SjGST), after adsorption onto aluminum hydroxide adjuvant, induced specific anti-SmCE immunoglobulin G (IgG) in all mice within 2 weeks of the second immunization. The IgG antibody response to rSmCE-SjGST was mainly of the IgG1 subclass. These results suggest that inactive forms of the antigen could be used to obtain the optimum immunogenic effects as a vaccine candidate against schistosomiasis. Mice immunized with the rSmCE-SjGST on alum had smaller mean worm burdens and lower tissue egg counts when compared with adjuvant alone- and recombinant SjGST-injected controls. The native SmCE was antigenically cross-reactive with homologous enzymes of Schistosoma haematobium and Schistosoma margrebowiei.

Item Type: Article
Additional Information: © 2017 Cambridge University Press. This is the accepted author manuscript (AAM). The final published version (version of record) will be available online via Cambridge University Press at https://www.cambridge.org/core/journals/parasitology - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: cercarial elastase, Schistosoma mansoni, enzyme, immunogenicity, vaccine, antigen, recombinant, GST fusion protein
Subjects: R Medicine > RZ Other systems of medicine
Divisions: Faculty of Natural Sciences > School of Life Sciences
Depositing User: Symplectic
Date Deposited: 09 May 2017 12:47
Last Modified: 17 May 2017 10:16
URI: http://eprints.keele.ac.uk/id/eprint/3376

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