Horrocks, PD ORCID: https://orcid.org/0000-0003-3981-0732, Russell, K and Emes, R (2015) Triaging informative cis -regulatory elements for the combinatorial control of temporal gene expression during Plasmodium falciparum intraerythrocytic development. Parasites and Vectors, 8 (1). p. 81.

[img]
Preview
Text
Russell P&V 2015.pdf
Available under License Creative Commons Attribution.

Download (1MB) | Preview

Abstract

Background
Over 2700 genes are subject to stage-specific regulation during the intraerythrocytic development of the human malaria parasite Plasmodium falciparum. Bioinformatic analyses have identified a large number of over-represented motifs in the 5′ flanking regions of these genes that may act as cis-acting factors in the promoter-based control of temporal expression. Triaging these lists to provide candidates most likely to play a role in regulating temporal expression is challenging, but important if we are to effectively design in vitro studies to validate this role.

Methods
We report here the application of a repeated search of variations of 5′ flanking sequences from P. falciparum using the Finding Informative Regulatory Elements (FIRE) algorithm.

Results
Our approach repeatedly found a short-list of high scoring DNA motifs, for which cognate specific transcription factors were available, that appear to be typically associated with upregulation of mRNA accumulation during the first half of intraerythrocytic development.

Conclusions
We propose these cis-trans interactions may provide a combinatorial promoter-based control of gene expression to complement more global mechanisms of gene regulation that can account for temporal control during the second half of intraerythrocytic development.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via BioMed Central at http://dx.doi.org/10.1186/s13071-015-0701-0 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: AP2 transcription factor, Bioinformatics, Cis-acting DNA motifs, Combinatorial control, Finding informative regulatory elements, Malaria, Stage-specific expression
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Depositing User: Symplectic
Date Deposited: 26 Mar 2015 12:18
Last Modified: 07 May 2019 13:34
URI: http://eprints.keele.ac.uk/id/eprint/371

Actions (login required)

View Item View Item