Smith, WA, Lam, K-P, Dempsey, KP, Mazzocchi-Jones, D, Richardson, JB and Yang, Y ORCID: https://orcid.org/0000-0002-4292-627X (2014) Label free cell tracking in 3-D tissue engineering constructs with high resolution imaging. Proceedings of SPIE, 8942.

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Abstract

Within the field of tissue engineering there is an emphasis on studying 3-D live tissue structures. Consequently, to investigate and identify cellular activities and phenotypes in a 3-D environment for all in vitro experiments, including shape, migration/proliferation and axon projection, it is necessary to adopt an optical imaging system that enables monitoring 3-D cellular activities and morphology through the thickness of the construct for an extended culture period without cell labeling. This paper describes a new 3-D tracking algorithm developed for Cell-IQ®, an automated cell imaging platform, which has been equipped with an environmental chamber optimized to enable capturing time-lapse sequences of live cell images over a long-term period without cell labeling. As an integral part of the algorithm, a novel auto-focusing procedure was developed for phase contrast microscopy equipped with 20x and 40x objectives, to provide a more accurate estimation of cell growth/trajectories by allowing 3-D voxels to be computed at high spatiotemporal resolution and cell density. A pilot study was carried out in a phantom system consisting of horizontally aligned nanofiber layers (with precise spacing between them), to mimic features well exemplified in cellular activities of neuronal growth in a 3-D environment. This was followed by detailed investigations concerning axonal projections and dendritic circuitry formation in a 3-D tissue engineering construct. Preliminary work on primary animal neuronal cells in response to chemoattractant and topographic cue within the scaffolds has produced encouraging results.

Item Type: Article
Additional Information: From Conference Volume 8942, Dynamics and Fluctuations in Biomedical Photonics XI Valery V. Tuchin; Kirill V. Larin; Martin J. Leahy; Ruikang K. Wang San Francisco, California, United States, February 01, 2014. Copyright 2014, Society of Photo Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, or modification of the contents of the publication are prohibited.
Uncontrolled Keywords: depth from focus, topographic surface, reconstruction, 2.5D imaging, multiscale, phase contrast microscopy, neuron proliferation
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Natural Sciences > School of Computing and Maths
Related URLs:
Depositing User: Symplectic
Date Deposited: 03 Aug 2017 10:19
Last Modified: 20 May 2019 13:24
URI: http://eprints.keele.ac.uk/id/eprint/3871

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