Sirbu, D, Butcher, JB, Waddell, PG, Andras, PE and Benniston, AC (2017) Locally Excited State - Charge Transfer State Coupled Dyes as Optically Responsive Neuron Firing Probes. Chemistry - A European Journal, 23 (58). pp. 14639-14649. ISSN 0947-6539

[img] Text
submitted_version_to_chemistry.docx - Accepted Version
Available under License Creative Commons Attribution Non-commercial.

Download (4MB)

Abstract

A selection of NIR‐optically responsive neuron probes was produced comprising of a donor julolidyl group connected to a BODIPY core and several different styryl and vinylpyridinyl derived acceptor moieties. The strength of the donor–acceptor interaction was systematically modulated by altering the electron withdrawing nature of the aryl unit. The fluorescence quantum yield was observed to decrease as the electron withdrawing effect of the aryl subunit increased in line with changes of the Hammett parameter. The effectiveness of these fluorophores as optically responsive dyes for neuronal imaging was assessed by measuring the toxicity and signal‐to‐noise ratio (SNR) of each dye. A great improvement of SNR was obtained when compared to the first‐generation BODIPY‐based voltage sensitive dyes with concomitant toxicity decrease. The mechanism for the optical response is disparate from conventional cyanine‐based dyes, opening up a new way to produce effective voltage sensitive dyes that respond well into the NIR region.

Item Type: Article
Additional Information: This is the accepted author manuscript (AAM). The final published version (version of record) is available online via Wiley at http://dx.doi.org/10.1002/chem.201703366 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: BODIPY, neural imaging, fluorescence, voltage sensitive dyes, photochemistry
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Natural Sciences > School of Computing and Maths
Depositing User: Symplectic
Date Deposited: 22 Aug 2017 11:20
Last Modified: 21 Aug 2018 01:30
URI: http://eprints.keele.ac.uk/id/eprint/3920

Actions (login required)

View Item View Item