Sharma, A and Wood, LD and Richardson, JB and Roberts, S and Kuiper, NJ (2007) Glycosaminoglycan profiles of repair tissue formed following autologous chondrocyte implantation differ from control cartilage. Arthritis Research & Therapy, 9 (4). R79 -?. ISSN 1478-6354

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Abstract

Currently, autologous chondrocyte implantation (ACI) is the most commonly used cell-based therapy for the treatment of isolated femoral condyle lesions of the knee. A small number of centres performing ACI have reported encouraging long-term clinical results, but there is currently a lack of quantitative and qualitative biochemical data regarding the nature of the repair tissue. Glycosaminoglycan (GAG) structure influences physiological function and is likely to be important in the long-term stability of the repair tissue. The objective of this study was to use fluorophore-assisted carbohydrate electrophoresis (FACE) to both quantitatively and qualitatively analyse the GAG composition of repair tissue biopsies and compare them with age-matched cadaveric controls. We used immunohistochemistry to provide a baseline reference for comparison. Biopsies were taken from eight patients (22 to 52 years old) 1 year after ACI treatment and from four cadavers (20 to 50 years old). FACE quantitatively profiled the GAGs in as little as 5 microg of cartilage. The pattern and intensity of immunostaining were generally comparable with the data obtained with FACE. In the ACI repair tissue, there was a twofold reduction in chondroitin sulphate and keratan sulphate compared with age-matched control cartilage. By contrast, there was an increase in hyaluronan with significantly shorter chondroitin sulphate chains and less chondroitin 6-sulphate in repair tissue than control cartilage. The composition of the repair tissue thus is not identical to mature articular cartilage.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via BioMed Central at https://doi.org/10.1186/ar2278 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: Adult, Cartilage, Articular, Chondrocytes, Chondroitin Sulfates, Electrophoresis, Polyacrylamide Gel, Fluorescent Dyes, Glycosaminoglycans, Humans, Hyaluronic Acid, Immunohistochemistry, Keratan Sulfate, Middle Aged, Tissue Transplantation, Transplantation, Autologous, Wound Healing
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
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Depositing User: Symplectic
Date Deposited: 25 Aug 2017 07:53
Last Modified: 25 Aug 2017 08:01
URI: http://eprints.keele.ac.uk/id/eprint/3930

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