Uche, FI and Abed, MN and Abdullah, MI and Drijfhout, FP and McCullagh, J and Claridge, TWD and Richardson, A and Li, W (2017) Isochondodendrine and 2[prime or minute]-norcocsuline: additional alkaloids from Triclisia subcordata induce cytotoxicity and apoptosis in ovarian cancer cell lines. RSC Advances, 7 (70). 44154 -44161. ISSN 2046-2069

[img]
Preview
Text
uche_rsc_adv_2017.pdf - Published Version
Available under License Creative Commons Attribution.

Download (979kB) | Preview

Abstract

Triclisia subcordata Oliv (Menispermeaceae) is used in herbal medicine for the treatment of cancer and other diseases in Africa. This study aims to isolate minor alkaloids present in this plant and assay their cytotoxic activities. Isochondodendrine and 2[prime or minute]-norcocsuline as two minor alkaloids together with the abundant cycleanine were isolated and identified by mass spectrometry and NMR spectroscopy. Both isochondodendrine and 2[prime or minute]-norcocsuline exhibited in vitro cytotoxicity in four ovarian cancer cell lines (A2780, IGROV-1, OVCAR-8, and OVCAR-4) with IC50 ranges of 3.5-17 [small mu ]M and 0.8-6.2 [small mu ]M respectively. These alkaloids showed mostly slightly weaker potencies when tested using normal human ovarian epithelial cells, IC50 = 10.5 +/- 1.2 [small mu ]M and 8.0 +/- 0.2 [small mu ]M for isochondodendrine and 2[prime or minute]-norcocsuline, respectively. The alkaloids induced apoptosis in ovarian cancer cells because they activated caspases 3/7, induced cleavage of PARP, increased the subG1 population in cell cycle analysis and increased Annexin V/propidium iodide staining. These observations suggest that isochondodendrine and 2[prime or minute]-norcocsuline contributing to the cytotoxic activity of T. subcordata may be suitable starting points for the future development of novel therapeutics to treat ovarian cancer.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via RSC at http://dx.doi.org/10.1039/C7RA08032H - please refer to any applicable terms of use of the publisher.
Subjects: Q Science > QD Chemistry > QD415 Biochemistry
Divisions: Faculty of Medicine and Health Sciences > School of Health and Rehabilitation
Depositing User: Symplectic
Date Deposited: 20 Sep 2017 14:13
Last Modified: 20 Sep 2017 14:14
URI: http://eprints.keele.ac.uk/id/eprint/4029

Actions (login required)

View Item View Item