Rotherham, M, Henstock, J, Quatachi, O and El Haj, AJ (2018) Remote regulation of magnetic particle targeted Wnt signalling for bone tissue engineering. Nanomedicine: Nanotechnology, Biology and Medicine, 14 (1). pp. 173-184.

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A El Haj - Remote regulation of magnetic particle targeted Wnt signalling for bone tissue engineering.pdf - Accepted Version
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Abstract

Wnt signaling is critically involved in the differentiation of human Mesenchymal Stem Cells (hMSC). Wnt proteins therefore have considerable therapeutic value, but are expensive and difficult to produce. UM206 is a synthetic peptide and ligand for the Wnt receptor Frizzled. Attachment of UM206 to magnetic nanoparticles (MNP) enables the ligand-MNP complex to be manipulated using magnetic fields, allowing control of Frizzled stimulation. Using this approach, Wnt signaling was activated in hMSC which resulted in Frizzled clustering, β-catenin translocalization and activation of TCF/LEF responsive transcription. During osteogenesis, UM206-MNP initiated localized mineralized matrix formation. Injection and magnetic stimulation of UM206-MNP-labeled MSC in ex vivo chick femurs resulted in increased mineralization which acted synergistically with addition of bone morphogenic protein 2 (BMP2) releasing micro-particles. As this facilitates external control over signal transduction, conjugated MNP technology has applications both as a research tool and for regulating tissue formation in clinical cell therapies.

Item Type: Article
Additional Information: This is the accepted author manuscript (AAM). The final published version (version of record) is available online via Elsevier at http://dx.doi.org/10.1016/j.nano.2017.09.008 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: Mesenchymal stem cells, Magnetic nanoparticles, Wnt signalling, bone tissue engineering
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Depositing User: Symplectic
Date Deposited: 29 Sep 2017 08:41
Last Modified: 18 Mar 2019 14:24
URI: http://eprints.keele.ac.uk/id/eprint/4060

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