Kizilors, A and Pickard, MR and Schulte, CE and Yacqub-Usman, K and McCarthy, NJ and Gan, S-U and Darling, D and Gäken, J and Williams, GT and Farzaneh, F (2017) Retroviral insertional mutagenesis implicates E3 ubiquitin ligase RNF168 in the control of cell proliferation and survival. Bioscience Reports. ISSN 0144-8463

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Abstract

The E3 ubiquitin ligase RNF168 is a ring finger protein that has previously been identified to play an important regulatory role in the repair of double-strand DNA breaks.  In the present study, an unbiased forward genetics functional screen in mouse granulocyte/ macrophage progenitor cell line FDCP1 has identified E3 ubiquitin ligase RNF168 as a key regulator of cell survival and proliferation. Our data indicate that RNF168 is an important component of the mechanisms controlling cell fate, not only in human and mouse haematopoietic growth factor-dependent cells, but also in the human breast epithelial cell line MCF-7. These observations therefore suggest that RNF168 provides a connection to key pathways controlling cell fate, potentially through interaction with PML nuclear bodies and/or epigenetic control of gene expression. Our study is the first to demonstrate a critical role for RNF168 in the in the mechanisms regulating cell proliferation and survival, in addition to its well-established role in DNA repair.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via Portland Press, Biochemical Society at http://dx.doi.org/10.1042/BSR20170843 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: Retroviral Insertional Mutagenesis, Ubiquitin, apoptosis, cell proliferation, cell survival
Subjects: Q Science > QD Chemistry > QD415 Biochemistry
Divisions: Faculty of Natural Sciences > School of Life Sciences
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Depositing User: Symplectic
Date Deposited: 19 Oct 2017 10:40
Last Modified: 19 Oct 2017 10:43
URI: http://eprints.keele.ac.uk/id/eprint/4119

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