Sidwick, KL, Johnson, AE, Adam, CD, Pereira, L and Thompson, DF (2017) The Use of Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry and Metabonomic Profiling to Differentiate Between Normally Slaughtered and Dead On Arrival Poultry Meat. Analytical Chemistry, 89 (22). pp. 12131-12136.

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The Use of Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry and Metabonomic Profiling to Differentiate Between Normally Slaughtered and Dead On Arrival Poultry Meat.docx - Accepted Version
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Abstract

Metabonomic profiling techniques, with established quality control methods, have been used to detect subtle metabolic differences in tissue that could aid in the discovery of fraud within the food industry. Liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) was utilized to acquire metabolic profiles of muscle, heart, and liver tissue from normally slaughtered and dead on arrival chickens. A workflow including XCMS Online for data processing and robust confirmatory statistics was used in order to differentiate between the two sample types. It was found that normally slaughtered and dead on arrival chicken can be differentiated based on the metabolic profile and multivariate analysis. Markers were found to be significantly different between the two sample types in all samples. With the use of the METLIN database and MS/MS analysis of chemical standards, sphingosine was identified as a marker in the muscle tissue samples which may offer potential for the detection of fraudulently processed chicken meat. The approach taken in this work has shown that it is possible to apply the described workflows to food fraud problems, with an objective of identifying key markers that could be investigated further to determine their usefulness for fraud detection.

Item Type: Article
Additional Information: This is the accepted author manuscript (AAM0. The final published version (version of record) will be available online via American Chemical Society at http://pubs.acs.org/journal/ancham - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: chemistry
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Natural Sciences > School of Chemical and Physical Sciences
Depositing User: Symplectic
Date Deposited: 27 Oct 2017 08:40
Last Modified: 18 Mar 2019 16:47
URI: http://eprints.keele.ac.uk/id/eprint/4134

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