Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy

Yang

doi:10.1016/j.ymeth.2017.10.010

Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy

Yang

Authors

Ying Yang y.yang@keele.ac.uk

Abstract

Cell mechanical behaviour is increasingly recognised as a central biophysical parameter in cancer and stem cell research, and methods of investigating their mechanical behaviour are therefore needed. We have developed a novel qualitative method based on quantitative phase imaging which is capable of investigating cell mechanical behaviour in real-time at cellular resolution using optical coherence phase microscopy (OCPM), and stimulating the cells non-invasively using hydrostatic pressure. The method was exemplified to distinguish between cells with distinct mechanical properties, and transient change induced by Cytochalasin D. We showed the potential of quantitative phase imaging to detect nanoscale intracellular displacement induced by varying hydrostatic pressure in microfluidic channels, reflecting cell mechanical behaviour. Further physical modelling is required to yield quantitative mechanical properties.

Acceptance Date	Oct 17, 2017
Publication Date	Mar 1, 2018
Journal	Methods
Print ISSN	1046-2023
Publisher	Elsevier
Pages	126-133
DOI	https://doi.org/10.1016/j.ymeth.2017.10.010
Keywords	optical coherence phase microscopy, mechanical behaviour, real-time monitoring, hydrostatic pressure, phase imaging
Publisher URL	http://dx.doi.org/10.1016/j.ymeth.2017.10.010