Popp, D, Loh, ND, Zorgati, H, Ghoshdastider, U, Liow, LT, Ivanova, MI, Larsson, M, DePonte, DP, Bean, R, Beyerlein, KR, Gati, C, Oberthuer, D, Arnlund, D, Brändén, G, Berntsen, P, Cascio, D, Chavas, LMG, Chen, JPJ, Ding, K, Fleckenstein, H, Gumprecht, L, Harimoorthy, R, Mossou, E, Sawaya, MR, Brewster, AS, Hattne, J, Sauter, NK, Seibert, M, Seuring, C, Stellato, F, Tilp, T, Eisenberg, DS, Messerschmidt, M, Williams, GJ, Koglin, JE, Makowski, L, Millane, RP, Forsyth, T, Boutet, S, White, TA, Barty, A, Chapman, H, Chen, SL, Liang, M, Neutze, R and Robinson, RC (2017) Flow-aligned, single-shot fiber diffraction using a femtosecond X-ray free-electron laser. Cytoskeleton (Hoboken), 74 (12). 472 - 481. ISSN 1949-3592

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Abstract

A major goal for X-ray free-electron laser (XFEL) based science is to elucidate structures of biological molecules without the need for crystals. Filament systems may provide some of the first single macromolecular structures elucidated by XFEL radiation, since they contain one-dimensional translational symmetry and thereby occupy the diffraction intensity region between the extremes of crystals and single molecules. Here, we demonstrate flow alignment of as few as 100 filaments (Escherichia coli pili, F-actin, and amyloid fibrils), which when intersected by femtosecond X-ray pulses result in diffraction patterns similar to those obtained from classical fiber diffraction studies. We also determine that F-actin can be flow-aligned to a disorientation of approximately 5 degrees. Using this XFEL-based technique, we determine that gelsolin amyloids are comprised of stacked β-strands running perpendicular to the filament axis, and that a range of order from fibrillar to crystalline is discernable for individual α-synuclein amyloids.

Item Type: Article
Additional Information: © 2017 The Authors Cytoskeleton Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Natural Sciences > School of Chemical and Physical Sciences
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Depositing User: Symplectic
Date Deposited: 19 Jan 2018 10:31
Last Modified: 19 Jan 2018 10:31
URI: http://eprints.keele.ac.uk/id/eprint/4385

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