Guerriero, G and Law, C and Stokes, I and Moore, KL and Exley, C (2018) Rough and tough. How does silicic acid protect horsetail from fungal infection? Journal of Trace Elements in Medicine and Biology, 47. 45 - 52. ISSN 0946-672X

[img] Text
C Exley - Rough and tough. How does silicic acid protect horsetail from fungal infection.pdf - Accepted Version
Restricted to Repository staff only until 31 January 2019.
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (16MB)


Abstract Horsetail (Equisetum arvense) plants grew healthily for 10 weeks under both Si-deficient and Si-replete conditions. After 10 weeks, plants grown under Si-deficient conditions succumbed to fungal infection. We have used NanoSIMS and fluorescence microscopy to investigate silica deposition in the tissues of these plants. Horsetail grown under Si-deficient conditions did not deposit identifiable amounts of silica in their tissues. Plants grown under Si-replete conditions accumulated silica throughout their tissues and especially in the epidermis of the outer side of the leaf and the furrow region of the stem where it was continuous and often, as a double layer suggestive of a barrier function. We have previously shown, both in vivo (in horsetail and thale cress) and in vitro (using an undersaturated solution of Si(OH)4), that callose is a “catalyst” of plant silica deposition. Here we support this finding by comparing the deposition of silica to that of callose and by showing that they are co-localized. We propose the existence of a synergistic mechanical protection by callose and silica against pathogens in horsetail, whereby the induction of callose synthesis and deposition is the first, biochemical line of defence and callose-induced precipitation of silica is the second, adventitious mechanical barrier.

Item Type: Article
Uncontrolled Keywords: Biogenic silica; NanoSIMS; Silicic acid; Callose; Horsetail; Fungal infection
Subjects: Q Science > QH Natural history
Q Science > QK Botany
Divisions: Faculty of Natural Sciences > School of Life Sciences
Related URLs:
Depositing User: Symplectic
Date Deposited: 15 Feb 2018 11:17
Last Modified: 15 Feb 2018 11:17

Actions (login required)

View Item View Item