Goncalves, A, Rotherham, M, Markides, H, Rodrigues, M, Reis, R, Gomes, M and El Haj, AJ (2018) Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation. Nanomedicine: Nanotechnology, Biology and Medicine, 14 (4). pp. 1149-1159. ISSN 1549-9634

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Abstract

Stem cell therapies hold potential to stimulate tendon regeneration and homeostasis, which is maintained in response to the native mechanical environment. Activins are members of the mechano-responsive TGF-β superfamily that participates in the regulation of several downstream biological processes. Mechanosensitive membrane receptors such as activin can be activated in different types of stem cells via magnetic nanoparticles (MNPs) through remote magnetic actuation resulting in cell differentiation. In this work, we target the Activin receptor type IIA (ActRIIA) in human adipose stem cells (hASCs), using anti-ActRIIA functionalized MNPs, externally activated through a oscillating magnetic bioreactor. Upon activation, the phosphorylation of Smad2/3 is induced allowing translocation of the complex to the nucleus, regulating tenogenic transcriptional responses. Our study demonstrates the potential remote activation of MNPs tagged hASCs to trigger the Activin receptor leading to tenogenic differentiation. These results may provide insights toward tendon regeneration therapies.

Item Type: Article
Uncontrolled Keywords: human adipose derived stem cells; magnetic nanoparticles; Activin A receptor; TGF-β/Smad2/3 signaling pathway; tendon tissue engineering
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Depositing User: Symplectic
Date Deposited: 20 Feb 2018 09:39
Last Modified: 23 Apr 2018 12:38
URI: http://eprints.keele.ac.uk/id/eprint/4491

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