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Coupling Plant-Derived Cyclotides to Metal Surfaces: An Antibacterial and Antibiofilm Study

Cao, Pan; Yang, Ying; Ijeoma Uche, Fidelia; Ruth Hart, Sarah; Li, Wen-Wu; Yuan, Chengqing

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Authors

Pan Cao

Fidelia Ijeoma Uche

Chengqing Yuan



Abstract

Modification of metal surfaces with antimicrobial peptides is a promising approach to reduce bacterial adhesion. Here, cyclic peptides or cycloids, possessing remarkable stability and antimicrobial activities, were extracted and purified from Viola philippica Cav., and identified using mass spectrometry. Cyclotides were subsequently utilized to modify stainless steel surfaces via polydopamine-mediated coupling. The resulting cyclotide-modified surfaces were characterized by Fourier transform infrared (FTIR) spectroscopy and contact angle analysis. The antibacterial capacity of these cyclotides against Staphylococcus aureus was assessed by Alamar blue assay. The antibiofilm capacity of the modified surfaces was assessed by crystal violet assay, and scanning electron microscopy (SEM). A composite of Kalata b1, Varv A, Viba 15 and Viba 17 (P1); Varv E (P2); and Viphi G (P3) were isolated and identified. FTIR analysis of the modified surfaces demonstrated that cyclotides bound to the surfaces and induced reduction of contact angles. Antimicrobial effects showed an order P3 > P1 and P2, with P3-treated surfaces demonstrating the strongest antibiofilm capacity. SEM confirmed reduced biofilm formation for P3-treated surfaces. This study provides novel evidence for cyclotides as a new class for development of antibacterial and antibiofilm agents.

Acceptance Date Mar 7, 2018
Publication Date Mar 9, 2018
Publicly Available Date Mar 29, 2024
Journal International Journal of Molecular Sciences
Print ISSN 1661-6596
Publisher MDPI
Pages 793 - 793
DOI https://doi.org/10.3390/ijms19030793
Keywords cyclotides: surface modification; antibiofilm; antibacterial; polydopamine
Publisher URL http://www.mdpi.com/1422-0067/19/3/793

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