Lu, L, Deegan, A, Musa, F, Xu, T and Yang, Y (2018) The Effects of Biomimetically Conjugated VEGF on Osteogenesis and Angiogenesis of MSCs (Human and Rat) and HUVECs Co-culture Models. Colloids and Surfaces B: Biointerfaces.

[img]
Preview
Text
Y Yang - The effects of biomometically conjugated VEGF on osteogenesis....pdf - Accepted Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (2MB) | Preview

Abstract

The purpose of this work was to investigate if the biomimetically conjugated VEGF and HUVECs co-culture could modulate the osteogenic and angiogenic differentiation of MSCs derived from rat and human bone marrow (rMSCs and hMSCs). After treated by ammonia plasma, Poly(lactic-co-glycolic acid) (PLGA) electrospun nanofibers were immobilized with VEGF through heparin to fulfil the sustained release. The proliferation capacity of rMSCs and hMSCs on neat PLGA nanofibers (NF) and VEGF immobilized NF (NF-VEGF) surfaces were assessed by CCK-8 and compared when MSCs were mono-cultured and co-cultured with HUVECs. The effect of VEGF and HUVECs co-culturing on osteogenic and angiogenic differentiation of rMSCs and hMSCs were investigated by calcium deposits and CD31 expression on NF and NF-VEGF surfaces. The results indicated that VEGF has been biomimetically immobilized onto PLGA nanofibers surface and kept sustained release successfully. The CD31 staining results showed that both VEGF and HUVECs co-culture could enhance the angiogenesis of rMSCs and hMSCs. However, the proliferation and osteogenic differentiation of MSCs when cultured with VEGF and HUVECs showed a species dependent response. Taken together, VEGF immobilization and co-culture with HUVECs promoted angiogenesis of MSCs, indicating a good strategy for vascularization in bone tissue engineering.

Item Type: Article
Additional Information: The final published version is available online at https://www.sciencedirect.com/science/article/pii/S092777651830273X
Uncontrolled Keywords: MSCs; HUVECs; Co-culture; Osteogenic; Angiogenic
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Depositing User: Symplectic
Date Deposited: 01 May 2018 09:02
Last Modified: 30 Apr 2019 01:30
URI: http://eprints.keele.ac.uk/id/eprint/4801

Actions (login required)

View Item View Item