Watkinson
Image detection of yeast Saccharomyces cerevisiae by Light-Addressable Potentiometric Sensors (LAPS)
Watkinson
Authors
Abstract
A light-addressable potentiometric sensor (LAPS) was developed for the image detection of multilayer yeast Saccharomyces cerevisiae. By modifying silicon-on-sapphire (SOS) with a monolayer of 1,8-nonadiyne, an inert LAPS substrate, insensitive to pH was obtained. Low gelling temperature agarose gel was shown to be an effective immobilization reagent for yeast cells in LAPS measurements without causing a background signal. The photocurrent images obtained were shown to be induced by the surface negative charge and the local impedance of the yeast cells.
| Acceptance Date | Aug 23, 2016 |
|---|---|
| Publication Date | Aug 24, 2016 |
| Journal | Electrochemistry Communications |
| Print ISSN | 1388-2481 |
| Publisher | Elsevier |
| Pages | 41 - 45 |
| DOI | https://doi.org/10.1016/j.elecom.2016.08.017 |
| Keywords | LAPSScanning Photo-induced Impedance Microscopy (SPIM)Cell imagingSaccharomyces cerevisiae |
| Publisher URL | https://doi.org/10.1016/j.elecom.2016.08.017 |
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