Weinberg, D, Adams, C and Chari, DM (2015) Deploying clinical grade magnetic nanoparticles with magnetic fields to magnetolabel neural stem cells in adherent versus suspension cultures. RSC Advances, 5 (54). pp. 43353-43360. ISSN 2046-2069

[img]
Preview
Text
D Chari - Deploying clinical grade magnetic nanoparticles with magnetic fields to magnetolabel neural stem cells in adherent versus suspension cultures.pdf - Accepted Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview

Abstract

Neural stem cells (NSCs) have a high therapeutic potential for patients with neurological disease/injury given their neuroregenerative and immunomodulatory capabilities. In recent years, magnetic nanoparticles (MNPs) have been used as contrast agents in translational studies, to track transplanted NSCs using non-invasive magnetic resonance imaging (MRI). However, NSC uptake of MNPs is inherently low in the absence of chemical/biological uptake enhancing strategies such as cell targeting peptides and transfection agents – approaches which may be cytotoxic and alter cellular physiology. By contrast, physical delivery strategies relying on magnetic assistive methods can safely enhance MNP uptake into multiple neural cell types. The utility of this approach has been demonstrated for gene delivery grade particles but their application in enhancing ‘magnetolabelling’ with clinical grade contrast agents has never been evaluated. Here, we show that applied oscillating magnetic fields can safely enhance the uptake of a clinical grade MNP (Lumirem/Ferumoxsil) into NSCs propagated as neurospheres (suspension cultures, the preferred format for transplantation) but offer limited benefit for monolayer (adherent) cultures. This physical delivery method therefore has potential to facilitate cell labelling for clinical therapies.

Item Type: Article
Additional Information: This is the accepted author manuscript (AAM). The final published version (version of record) is available online via Royal Society of Chemistry at http://dx.doi.org/10.1039/C5RA07481A Please refer to any applicable terms of use of the publisher.
Subjects: R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Depositing User: Symplectic
Date Deposited: 14 May 2015 10:03
Last Modified: 12 Jun 2018 08:40
URI: http://eprints.keele.ac.uk/id/eprint/514

Actions (login required)

View Item View Item