Derricott, H, Luu, L, Fong, WY, Hartley, CS, Johnston, LJ, Armstrong, SD, Randle, N, Duckworth, CA, Campbell, BJ, Wastling, JM and Coombes, JL (2018) Developing a 3D intestinal epithelium model for livestock species. Cell and Tissue Research. ISSN 1432-0878

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Abstract

The in vitro 3D culture of intestinal epithelium is a valuable resource in the study of its function. Organoid culture exploits stem cells’ ability to regenerate and produce differentiated epithelium. Intestinal organoid models from rodent or human tissue are widely available whereas large animal models are not. Livestock enteric and zoonotic diseases elicit significant morbidity and mortality in animal and human populations. Therefore, livestock species-specific models may offer novel insights into host-pathogen interactions and disease responses. Bovine and porcine jejunum were obtained from an abattoir and their intestinal crypts isolated, suspended in Matrigel, cultured, cryopreserved and resuscitated. ‘Rounding’ of crypts occurred followed by budding and then enlargement of the organoids. Epithelial cells were characterised using immunofluorescent staining and confocal microscopy. Organoids were successfully infected with Toxoplasma gondii or Salmonella typhimurium. This 3D organoid model offers a long-term, renewable resource for investigating species-specific intestinal infections with a variety of pathogens.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via Springer at http://doi.org/10.1007/s00441-018-2924-9 - please refer to any applicable terms of use of the publisher.
Subjects: Q Science > QH Natural history
Divisions: Faculty of Natural Sciences > School of Life Sciences
Depositing User: Symplectic
Date Deposited: 28 Sep 2018 09:20
Last Modified: 28 Sep 2018 09:20
URI: http://eprints.keele.ac.uk/id/eprint/5376

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