Henstock, JR, Rotherham, M and El Haj, AJ (2018) Magnetic ion channel activation of TREK1 in human mesenchymal stem cells using nanoparticles promotes osteogenesis in surrounding cells. Journal of Tissue Engineering, 9. 2041731418808695 - ?. ISSN 2041-7314

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Abstract

Magnetic ion channel activation technology uses superparamagnetic nanoparticles conjugated with targeting antibodies to apply mechanical force directly to stretch-activated ion channels on the cell surface, stimulating mechanotransduction and downstream processes. This technique has been reported to promote differentiation towards musculoskeletal cell types and enhance mineralisation. Previous studies have shown how mesenchymal stem cells injected into a pre-mineralised environment such as a foetal chick epiphysis, results in large-scale osteogenesis at the target site. However, the relative contributions of stem cells and surrounding host tissue has not been resolved, that is, are the mesenchymal stem cells solely responsible for the observed mineralisation or do mechanically stimulated mesenchymal stem cells also promote a host-tissue mineralisation response? To address this, we established a novel two-dimensional co-culture assay, which indicated that magnetic ion channel activation stimulation of human mesenchymal stem cells does not significantly promote migration but does enhance collagen deposition and mineralisation in the surrounding cells. We conclude that one of the important functions of injected human mesenchymal stem cells is to release biological factors (e.g., cytokines and microvesicles) which guide the surrounding tissue response, and that remote control of this signalling process using magnetic ion channel activation technology may be a useful way to both drive and regulate tissue regeneration and healing.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via Sage Publications at http://doi.org/10.1177/2041731418808695 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: magnetic nanoparticles, mesenchymal stem cell, paracrine, stretch-activated ion channel, tissue engineering
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
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Depositing User: Symplectic
Date Deposited: 23 Nov 2018 11:18
Last Modified: 23 Nov 2018 11:19
URI: http://eprints.keele.ac.uk/id/eprint/5523

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