Adams, C.F., Delaney, AM, Carwardine, DR, Tickle, J, Granger, N and Chari, DM (2018) Nanoparticle-Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices. Macromolecular Bioscience. e1800389 - ?. ISSN 1616-5195

[img] Text
MABI_OEChydrogelpaper_Communications_revisedNov18_clean.pdf - Accepted Version
Restricted to Repository staff only until 4 December 2019.
Available under License Creative Commons Attribution Non-commercial.

Download (849kB)

Abstract

A recent clinical trial proves that autologous olfactory mucosal cell (OMC) transplantation improves locomotion in dogs with naturally occurring spinal injuries comparable to human lesions. However, not all dogs respond to the treatment, likely due to the transplantation procedures involving injections of cell suspensions that are associated with cell death, uneven cell distribution, and cell washout. Encapsulating cells in protective hydrogel matrices offers a tissue engineering solution to safely achieve 3D growth of viable transplant cells for implantation into injury sites, to improve regenerative outcomes. It is shown for the first time that canine OMCs (cOMCs) can be propagated with high viability in 3D collagen matrices. Further, a method to incorporate cOMCs pre-labeled with clinical-grade iron oxide nanoparticles into the constructs is described. Intraconstruct labeled cells are visualized using magnetic resonance imaging, offering substantial promise for in vivo tracking of cOMCs delivered in protective matrices.

Item Type: Article
Additional Information: This is the accepted author manuscript (AAM). The final published version (version of record) is available online via Wiley at https://doi.org/10.1002/mabi.201800389 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: canine olfactory mucosal cell, cell transplantation, hydrogel, magnetic nanoparticle, spinal injury
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 07 Dec 2018 12:18
Last Modified: 10 Dec 2018 15:39
URI: http://eprints.keele.ac.uk/id/eprint/5556

Actions (login required)

View Item View Item