Geng, R, Furness, DN, Muraleedharan, CK, Zhang, J, Dabdoub, A, Lin, V and Xu, S (2018) The microRNA-183/96/182 Cluster is Essential for Stereociliary Bundle Formation and Function of Cochlear Sensory Hair Cells. Scientific Reports, 8 (1). 18022 - ?. ISSN 2045-2322

[img]
Preview
Text
20190110_s41598-018-36894-z.pdf - Published Version
Available under License Creative Commons Attribution.

Download (2MB) | Preview

Abstract

The microRNA (miR)-183/96/182 cluster plays important roles in the development and functions of sensory organs, including the inner ear. Point-mutations in the seed sequence of miR-96 result in non-syndromic hearing loss in both mice and humans. However, the lack of a functionally null mutant has hampered the evaluation of the cluster's physiological functions. Here we have characterized a loss-of-function mutant mouse model (miR-183CGT/GT), in which the miR-183/96/182 cluster gene is inactivated by a gene-trap (GT) construct. The homozygous mutant mice show profound congenital hearing loss with severe defects in cochlear hair cell (HC) maturation, alignment, hair bundle formation and the checkboard-like pattern of the cochlear sensory epithelia. The stereociliary bundles retain an immature appearance throughout the cochlea at postnatal day (P) 3 and degenerate soon after. The organ of Corti of mutant newborn mice has no functional mechanoelectrical transduction. Several predicted target genes of the miR-183/96/182 cluster that are known to play important roles in HC development and function, including Clic5, Rdx, Ezr, Rac1, Myo1c, Pvrl3 and Sox2, are upregulated in the cochlea. These results suggest that the miR-183/96/182 cluster is essential for stereociliary bundle formation, morphogenesis and function of the cochlear HCs.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via Nature Publishing Group at http://doi.org/10.1038/s41598-018-36894-z - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: inner ear, hearing, sensory organs
Subjects: Q Science > QM Human anatomy
Q Science > QP Physiology
Divisions: Faculty of Natural Sciences > School of Life Sciences
Related URLs:
Depositing User: Symplectic
Date Deposited: 10 Jan 2019 13:44
Last Modified: 10 Jan 2019 13:44
URI: http://eprints.keele.ac.uk/id/eprint/5662

Actions (login required)

View Item View Item