Adesina-Georgiadis, KN, Gray, N, Plumb, RS, Thompson, DF, Holmes, E, Nicholson, JK and Wilson, ID (2018) The Metabolic Fate and Effects of 2-Bromophenol in Male Sprague-Dawley Rats. Xenobiotica. 1 -22. ISSN 1366-5928

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Abstract

1. The metabolic fate and urinary excretion of 2-bromophenol, a phenolic metabolite of bromobenzene, was investigated in male Sprague Dawley rats following single intraperitoneal doses at either 0, 100 or 200 mg/kg. 2. Urine was collected for seven days and samples analysed using 1H NMR spectroscopy, inductively coupled plasma (ICP)MS, and UPLC-MS. 3. 1H NMR spectroscopy of the urine samples showed that, at these doses, 2-bromophenol had little effect on endogenous metabolite profiles, supporting histopathology and clinical chemistry data which showed no changes associated with the administration of 2-bromophenol at these doses. 4. The use of ICP-MS Provided a means for the selective detection and quantification of bromine-containing species and showed that between 15 and 30% of the dose was excreted via the urine over the 7 days of the study for both the 100 and 200 mg doses respectively. 6. The bulk of the excretion of Br-containing material had occurred by 8 hr post administration. UPLC-MS of urine revealed a number of metabolites of 2-bromophenol, with 2-bromophenol glucuronide and 2-bromophenol sulphate identified as the major species. A number of minor hydroxylated metabolites were also detected as their glucuronide, sulphate or O-methyl conjugates. There was no evidence for the production of reactive metabolites.

Item Type: Article
Additional Information: This is the accepted author manuscript (AAM). The final published version (version of record) is available online via Taylor & Francis at https://doi.org/10.1080/00498254.2018.1559376 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: 2-Bromobenzene, metabolism, nephrotoxicity, metabonomics
Subjects: Q Science > QH Natural history
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Depositing User: Symplectic
Date Deposited: 22 Jan 2019 14:59
Last Modified: 22 Jan 2019 15:03
URI: http://eprints.keele.ac.uk/id/eprint/5717

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