Waldie, S, Lind, TK, Browning, K, Moulin, M, Haertlein, M, Forsyth, VT, Luchini, A, Strohmeier, GA, Pichler, H, Maric, S and Cárdenas, M (2017) Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine. Langmuir, 34 (1). 472 - 479. ISSN 1520-5827

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Abstract

Cholesterol is an essential component of mammalian membranes and is known to induce a series of physicochemical changes in the lipid bilayer. Such changes include the formation of liquid-ordered phases with an increased thickness and a configurational order as compared to liquid-disordered phases. For saturated lipid membranes, cholesterol molecules localize close to the lipid head group-tail interface. However, the presence of polyunsaturated lipids was recently shown to promote relocation of cholesterol toward the inner interface between the two bilayer leaflets. Here, neutron reflection is used to study the location of cholesterol (both non-deuterated and per-deuterated versions are used) within supported lipid bilayers composed of a natural mixture of phosphatidylcholine (PC). The lipids were produced in a genetically modified strain of Escherichia coli and grown under specific deuterated conditions to give an overall neutron scattering length density (which depends on the level of deuteration) of the lipids matching that of D2O. The combination of solvent contrast variation method with specific deuteration shows that cholesterol is located closer to the lipid head group-tail interface in this natural PC extract rather than in the center of the core of the bilayer as seen for very thin or polyunsaturated membranes.

Item Type: Article
Additional Information: The final published version of this publication is available online at http://pubs.acs.org/doi/10.1021/acs.langmuir.7b02716
Uncontrolled Keywords: cholesterol
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Natural Sciences > School of Life Sciences
Related URLs:
Depositing User: Symplectic
Date Deposited: 05 Feb 2019 15:47
Last Modified: 05 Feb 2019 15:47
URI: http://eprints.keele.ac.uk/id/eprint/5765

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