Ahmad, N, Colak, B, Zhang, D-W, Gibbs, MJ, Watkinson, M ORCID: https://orcid.org/0000-0003-1877-5667, Becer, CR, Gautrot, JE and Krause, S (2019) Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase. Sensors, 19 (7).

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Abstract

Peptide cross-linked poly(ethylene glycol) hydrogel has been widely used for drug delivery and tissue engineering. However, the use of this material as a biosensor for the detection of collagenase has not been explored. Proteases play a key role in the pathology of diseases such as rheumatoid arthritis and osteoarthritis. The detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a protease biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film and demonstrated for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals, and their degradation in the presence of collagenase was monitored using a quartz crystal microbalance (QCM). The biosensor was shown to respond to concentrations between 2 and 2000 nM in less than 10 min with a lower detection limit of 2 nM.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via MDPI AG at https://doi.org/10.3390/s19071677 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: protease biosensor; collagenase; poly(ethylene glycol) norbornene; QCM; hydrogel degradation; peptide cross-links; click chemistry
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Natural Sciences > School of Chemical and Physical Sciences
Depositing User: Symplectic
Date Deposited: 16 Apr 2019 15:27
Last Modified: 16 Apr 2019 15:27
URI: http://eprints.keele.ac.uk/id/eprint/6195

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