Drusco, A, Fadda, P, Nigita, G, Fassan, M, Bottoni, A, Gardiman, MP, Sacchi, D, Calore, F, Carosi, M, Antenucci, A, Casini, B, Kelani, H, Pescarmona, E, Di Leva, G, Zanesi, N, Berger, MS and Croce, CM (2018) Circulating Micrornas Predict Survival of Patients with Tumors of Glial Origin. EBioMedicine, 30. 105 -112.

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Abstract

The World Health Organization has recently introduced molecular prognostic-diagnostic biomarkers in the classification of Central Nervous System (CNS) tumors. In order to characterize subclasses of tumors that cannot find a precise location in the current classification, and, or cannot be tested because of scant material, it is important to find new molecular biomarkers in tissue and, or biological fluid samples. In this study, we identified serum microRNAs that could serve as biomarkers for the diagnosis and prognosis of patients with tumors of glial origin. We retrospectively analyzed microRNA expression in the serum extracellular vesicles of patients with tumors of glial origin. Extracellular vesicles RNA was analyzed by Nanostring. qRT-PCR confirmed 6 overexpressed microRNAs: hsa-miR-4443, hsa-miR-422a, hsa-miR-494-3p, hsa-miR-502-5p, hsa-miR-520f-3p, and hsa-miR-549a. Hsa-miR-4443 was the only microRNA that showed significant differences in most comparisons. In situ hybridization (ISH), confirmed that our signature was mostly expressed in cancer cells. Importantly, hsa-miR-549a and hsa-miR-502-5p expression predicted prognosis in patients with tumors of glial origin. Although more studies are needed, we demonstrated that serum vesicles microRNA profiles are promising diagnostic and prognostic molecular biomarkers that will find an actual application in the clinical practice of CNS tumors.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via Elsevier at https://doi.org/10.1016/j.ebiom.2018.03.022 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: microRNA, CNS tumors, Serum, Biomarkers, Diagnosis, Prognosis
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 26 Apr 2019 13:39
Last Modified: 26 Apr 2019 13:46
URI: http://eprints.keele.ac.uk/id/eprint/6211

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