Skip to main content

Research Repository

Advanced Search

Loss of miR-204 expression is a key event in melanoma

Galasso, Marco; Morrison, Carl; Minotti, Linda; Corrà, Fabio; Zerbinati, Carlotta; Agnoletto, Chiara; Baldassari, Federica; Fassan, Matteo; Bartolazzi, Armando; Vecchione, Andrea; Nuovo, Gerard J.; Di Leva, Gianpiero; D’Atri, Stefania; Alvino, Ester; Previati, Maurizio; Nickoloff, Brian J.; Croce, Carlo M.; Volinia, Stefano

Loss of miR-204 expression is a key event in melanoma Thumbnail


Authors

Marco Galasso

Carl Morrison

Linda Minotti

Fabio Corrà

Carlotta Zerbinati

Chiara Agnoletto

Federica Baldassari

Matteo Fassan

Armando Bartolazzi

Andrea Vecchione

Gerard J. Nuovo

Stefania D’Atri

Ester Alvino

Maurizio Previati

Brian J. Nickoloff

Carlo M. Croce

Stefano Volinia



Abstract

Cutaneous melanoma (CM) is a malignancy with increasing occurrence. Its microRNA repertoire has been defined in a number studies, leading to candidates for biological and clinical relevance: miR-200a/b/c, miR-203, miR-205, miR-204, miR-211, miR-23b and miR-26a/b. Our work was aimed to validate the role of these candidate miRNAs in melanoma, using additional patients cohorts and in vitro cultures. miR-26a, miR-204 and miR-211 were more expressed in normal melanocytes, while miR-23b, miR-200b/c, miR-203 and miR-205 in epidermis and keratinocytes. None of the keratinocyte-related miRNAs was associated with any known mutation or with clinical covariates in melanoma. On the other hand, the loss of miR-204 was enriched in melanomas with NRAS sole mutation (Fisher exact test, P =?0.001, Log Odds?=?1.67), and less frequent than expected in those harbouring CDKN2A mutations (Fisher exact test, P =?0.001, Log Odds -?1.09). Additionally, miR-204 was associated with better prognosis in two independent melanoma cohorts and its exogenous expression led to growth impairment in melanoma cell lines. Thus, miR-204 represents a relevant mechanism in melanoma, with potential prognostic value and its loss seems to act in the CDKN2A pathway, in cooperation with NRAS.

Journal Article Type Article
Acceptance Date Feb 22, 2018
Publication Date Mar 9, 2018
Publicly Available Date Mar 29, 2024
Journal Molecular Cancer
Print ISSN 1476-4598
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 17
Article Number 71
DOI https://doi.org/10.1186/s12943-018-0819-8
Keywords BRAF, Breslow, CDKN2A, Keratinocyte, Melanocyte, Melanoma, NRAS, Non coding rna, Somatic alterations, microRNA, Biomarkers, Tumor, Female, Gene Expression, Regulation, Neoplastic, Gene Knockdown Techniques, Humans, Male, MicroRNAs, Mutation, Prognosis
Publisher URL http://doi.org/10.1186/s12943-018-0819-8

Files




You might also like



Downloadable Citations