Galasso, M, Morrison, C, Minotti, L, Corrà, F, Zerbinati, C, Agnoletto, C, Baldassari, F, Fassan, M, Bartolazzi, A, Vecchione, A, Nuovo, GJ, Di Leva, G, D'Atri, S, Alvino, E, Previati, M, Nickoloff, BJ, Croce, CM and Volinia, S (2018) Loss of miR-204 expression is a key event in melanoma. Molecular Cancer, 17 (1). 71 -?.

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Abstract

Cutaneous melanoma (CM) is a malignancy with increasing occurrence. Its microRNA repertoire has been defined in a number studies, leading to candidates for biological and clinical relevance: miR-200a/b/c, miR-203, miR-205, miR-204, miR-211, miR-23b and miR-26a/b. Our work was aimed to validate the role of these candidate miRNAs in melanoma, using additional patients cohorts and in vitro cultures. miR-26a, miR-204 and miR-211 were more expressed in normal melanocytes, while miR-23b, miR-200b/c, miR-203 and miR-205 in epidermis and keratinocytes. None of the keratinocyte-related miRNAs was associated with any known mutation or with clinical covariates in melanoma. On the other hand, the loss of miR-204 was enriched in melanomas with NRAS sole mutation (Fisher exact test, P = 0.001, Log Odds = 1.67), and less frequent than expected in those harbouring CDKN2A mutations (Fisher exact test, P = 0.001, Log Odds - 1.09). Additionally, miR-204 was associated with better prognosis in two independent melanoma cohorts and its exogenous expression led to growth impairment in melanoma cell lines. Thus, miR-204 represents a relevant mechanism in melanoma, with potential prognostic value and its loss seems to act in the CDKN2A pathway, in cooperation with NRAS.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via BioMed Central at http://doi.org/10.1186/s12943-018-0819-8 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: BRAF, Breslow, CDKN2A, Keratinocyte, Melanocyte, Melanoma, NRAS, Non coding rna, Somatic alterations, microRNA, Biomarkers, Tumor, Female, Gene Expression, Regulation, Neoplastic, Gene Knockdown Techniques, Humans, Male, MicroRNAs, Mutation, Prognosis
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 26 Apr 2019 13:47
Last Modified: 26 Apr 2019 13:54
URI: http://eprints.keele.ac.uk/id/eprint/6212

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