Santis, ED, Shardlow, E, Stellato, F, Proux, O, Rossi, G, Exley, C and Morante, S (2019) X-Ray Absorption Spectroscopy Measurements of Cu-ProIAPP Complexes at Physiological Concentrations. Condensed Matter, 4 (1). 13 -?.

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Abstract

The amyloidogenic islet amyloid polypeptide (IAPP) and the associated pro-peptide ProIAPP1–48 are involved in cell death in type 2 diabetes mellitus. It has been observed that interactions of this peptide with metal ions have an impact on the cytotoxicity of the peptides as well as on their deposition in the form of amyloid fibrils. In particular, Cu(II) seems to inhibit amyloid fibril formation, thus suggesting that Cu homeostasis imbalance may be involved in the pathogenesis of type 2 diabetes mellitus. We performed X-ray Absorption Spectroscopy (XAS) measurements of Cu(II)-ProIAPP complexes under near-physiological (10 μM), equimolar concentrations of Cu(II) and peptide. Such low concentrations were made accessible to XAS measurements owing to the use of the High Energy Resolved Fluorescence Detection XAS facility recently installed at the ESRF beamline BM16 (FAME-UHD). Our preliminary data show that XAS measurements at micromolar concentrations are feasible and confirm that ProIAPP1–48-Cu(II) binding at near-physiological conditions can be detected.

Item Type: Article
Additional Information: This is the final published version of the article (version of record). It first appeared online via MDPI at https://doi.org/10.3390/condmat4010013 - please refer to any applicable terms of use of the publisher.
Uncontrolled Keywords: X-ray absorption spectroscopy, amylin, high energy resolution fluorescence detection
Subjects: Q Science > QH Natural history
Divisions: Faculty of Natural Sciences > School of Life Sciences
Depositing User: Symplectic
Date Deposited: 29 Apr 2019 10:04
Last Modified: 29 Apr 2019 10:05
URI: http://eprints.keele.ac.uk/id/eprint/6230

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